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Proteins, carbohydrates, fats

Source: licencja: CC 0.

Link to the lesson

Before you start you should know
  • human is a heterotrophic organism;

  • nourishment means absorbing nutrients which are a building material, an energy source and a reserve supply.

You will learn
  • to talk about types, significance and sources of nutrients;

  • to present the functions of fiber;

  • to describe the functions of complete and incomplete proteins in the correct functioning of our organism;

  • to justify the necessity for eating fruit and vegetables regularly;

  • to plan and conduct experiments that allow to detect proteins, sugars, fats that are in food and interpret the results of these experiments.

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Nagranie dźwiękowe abstraktu

Proteins

For organisms to be able to fulfill all vital functions, maintain (or increase) their mass and reproduce, they need various substances. The most important are organic compounds: proteins, sugars, fats, vitamins, and inorganic compounds – water and mineral salts.

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Energy value of nutrients
Source: Dariusz Adryan, licencja: CC BY 3.0.

Proteins are absorbed with food and used mainly to build the organism. They constantly renew used cell elements, they are an ingredient of various secretions of the cells, and in childhood and puberty they create new cells, allowing our organism to grow and develop. In addition, they may be used as an energy ingredient, but this happens rarely (e.g. in periods of hunger) and leads to, e.g. muscle atrophy. Other functions of proteins are presented in the table.

Functions of proteins

Examples of proteins and their functions

building

actin, myosin – ingredients of muscle cells

ingredients of cell membranes, hair, nails, bones

keratin, collagen – ingredients of skin

enzymatic

pepsin – digesting proteins

digestion of other ingredients of food

other digestive enzyms

immunological

antibodies – fight against bacteria and viruses

transport

hemoglobin – it transports oxygen and carbon dioxide

hormonal

insulin – it increases the transport of sugar from the blood to the cells

receptive

rhodopsin – reacting to light

receiving other stimuli

other receptive proteins

In the digestive tract, plant proteins and animal proteins are digested into amino acids, from which our body constructs proteins as needed. Part of the 20 amino acids included in proteins can be produced by our body as a result of the digestion of proteins, sugars and fats. These are endogenous amino acidsendogenous amino acidsendogenous amino acids. Other amino acids must be taken from food, as our body cannot create them. These are exogenous amino acidsexogenous amino acidsexogenous amino acids.

Valuable proteins that include all the necessary amino acids are present in animal products, such as milk and dairy, in meat and in fish. They are defined as a standard. Seeds of legumes are characterized by high amount of plant proteins, called sub‑standard proteins. In case of a plant based diet (vegetarian diet), only a combination of selected plant products that underwent a specific preparation is able to provide our body with all necessary amino acids. Giving up on eating meat may lead to deficiencies of some of them. Without them, specific proteins will not be created, which may cause to inhibit growth, can lead to dystrophy of muscles and of other tissues, to anaemia and decalcification of bones.

The daily amount of protein for an adult is around 1 gram per 1 kilogram of body weight. In people who work physically, pregnant women, children and teenagers who are still growing, the amount is twice as high.

Task 1

Explain why pregnant women should increase the amount of proteins they consume.

Carbohydrates (sugars)

The main source of carbohydrates are mainly products of plant origin and, to smaller extent, products of animal origin. By eating them, we provide our body mainly with:

  • polysaccharides, like starchstarchstarch – plant spare sugar, glycogenglycogenglycogen – animal spare sugar;

  • disaccharides, like sucrose – sugar in fruit, cereals, available in crystal sugar form or powdered form; lactose – sugar in milk and dairy;

  • monosaccharides, such as fructose – fruit sugar.

Polysaccharides and disaccharides in the digestive tract are digested by digestive enzymes until they become monosaccharides, from which glucose has the most important role. It is the main source of energy used by cells. Some time after eating, its concentration in blood increases, and as the digestion progresses, it is maintained at a rather even level. It gives us the sense of satiety.

Glucose present, for example, in sweets, malt sugar and jams does not require digestion, meaning it enters the bloodstream really quick. Glucose is the product of digestion of starch, e.g. in wholemeal bread, is gradually released into the blood and, for some time, it stays at a certain level. When glucose level in the blood drops, we feel the urge to eat. In case of eating sweets, we start feeling hungry shortly after we finish eating. This may be the cause of eating more portions of food and can lead to obesity.

If monosaccharides are not used as a source of energy (e.g. during physical exercises or intense mental work), their excess is partially stored in the liver and muscles as a spare sugar glycogen. The rest is transformed into fat and, in that form, is stored in fat cells, e.g. in the subcutaneous fat tissue.

The most popular source of polysaccharides of plant origins are potatoes and cereals: wheat, rye, barley, oat, corn and rice. Flour used to bake bread and make pasta (as well as rice) is very often purified, that is made from grains that lack tegument. Such flour is white, just like the bread we get from it. Bleaching flour deprives it of B group vitamins, calcium, phosphorus, iron and fiber. FiberfiberFiber includes cellulose (polysaccharide constructing cell walls in plants), which is not digested by human digestive tract. Fiber does not have any nutrients or energy values. However, it has a great health significance, because it speeds up the movement of the food content in the intestine, prevents constipation and colon cancer. It expands inside the stomach and gives you the feeling of satiety, it binds the surplus of hydrochloric acid and lowers the level of cholesterol. It is present in large amounts in cereals, fruit and vegetables. This is why we should eat wholemeal bread and pasta, dark rice and fruit with peel on (which is rich in fiber).

The type and amount of carbohydrates we eat has an important influence on our health. Too much of them can lead to caries, obesity and diabetes. Caring for the correct condition of our body, we should limit the use of sucrose available mainly in form of sugar and sweets. Young people and adults should consume carbohydrates in the amount of 3‑4 grams per kilogram of body weight a day, depending on their activity. In addition, they should consume 30‑50 grams of fibers.

Task 2

Prove and give 2 arguments why eating food rich in fiber prevents obesity.

Experiment 1

Detecting starch in food.

You will need
  • iodine,

  • dropping pipette,

  • potato starch, a slice of raw potato, a slice of raw cucumber, apple, a piece of cottage cheese, a slice of banana, a hot dog sausage,

  • 1 big plate with separated samples.

Instruction
  1. Check the result of the reaction between iodine and starch. Add a drop of iodine to potato starch (substance which we know for sure that consists mainly of starch). As a result of the reaction with starch, brown‑reddish iodine turns dark blue.

  2. Put a drop of iodine into each of the remaining products and observe the colour.

Summary

If iodine changes colour from brown to dark blue, this means the product we are testing has starch in it.

Observation 1

To assess how bran behaves in contact with water.

You will need
  • water at 37°C,

  • 50 ml of rye bran, wheat bran and oat bran,

  • 3 beakers with the capacity of 200 ml,

  • 3 dishes with water, with the capacity of 50 ml.

Instruction
  1. Put bran into the beakers. Mark the level where the bran reaches to.

  2. Pour equal amount of warm water (50 ml) into all the bran beakers at the same time.

  3. Put the beakers aside for 20 minutes.

  4. After this time, mark the level where the bran reaches to.

  5. Observe, which beaker absorbed most water.

Summary

The main ingredient of bran is fiber, which soaks water and swells.

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Grafika przedstawia trzy modele, struktury białka, aminokwasów i tłuszczu. Modele zostały zobrazowane jako układ połączonych ze sobą niewielkich kropek. Struktura białka - protein structure to kolorowe kropki połączone ze sobą nieregularnie, dodatkowo przeplecione szarą wstęgą obrazującą helisę alfa. To struktura drugorzędowa białka secondary structure. Aminokwasy amino acid sequence zostały przedstawione jako połączone niczym w łańcuchu jednokolorowe kropki. Model komórki tłuszczu - model of fat molecule zobrazowano jako układ regularnie połączonych zielonych i niebieskich kropek.
Selected models of structures of protein and fat
Source: Thomas Shafee, licencja: CC BY-SA 4.0.

Fats

Fats provide twice as much energy than sugars, which is why they are the energy reserve of our body used mainly during an intense physical effort. Besides this, they are the ingredient of biological membranes, hormones (e.g. sex hormones) and facilitate the absorption of vitamins A, D, E and K. They are also stored in the subcutaneous fat tissue where they form insulating layer, and by covering certain internal organs (e.g. kidneys), they protect them from injuries and impact. We eat fats in form of fats defined as hard fats (butter, margarine), oils (e.g. sunflower seed oil, rape seed oil), and together with fried foods, e.g. scrambled eggs, fries and chips.

Simplifying it, we can say that a fat particle consists of glycerolglycerolglycerol and 3 molecules of fatty acidsfatty acidsfatty acids. The nutrient value of fats is decided by the amount and type of fatty acids. The most beneficial fats for our health found in seawater fish and plants are rich in fatty acids defined as unsaturated. Unsaturated fatty acids lower the level of cholesterolcholesterolcholesterol in the blood, lessening the risk of arteriosclerosis. The most important are the unsaturated fatty acids omega‑6, present in plant oils, as well as omega‑3, present in fish. Those acids help the nervous system to develop, which is why they should be an important element of a child's diet. They also facilitate wound healing and lower blood pressure.

Pork, mutton and beef fats contain saturated fatty acids, which can increase the level of cholesterol. You need to remember that cholesterol has also beneficial functions in our body – it is the component of the casing of neural protrusions, bile, vitamin D and some hormones. Surplus of this compound is stored in the walls of the blood vessels, which makes them more and more narrow and restrict blood flow.

Trans fatty acids are dangerous for our health. They can be found in hardened fats (e.g. margarine) used in frying and baking. Those acids cause the arteriosclerosis to develop, they are the cause of embolisms in blood vessels and heart attacks. Even beneficial vegetable oils, after undergoing incorrect or too long (sometimes repeated) heat treatment, are transformed and release harmful fatty acids. Because of that, we should eliminate from our daily diet fast food (chips, fries, fried food) and substitute them with boiled or steamed products. An adult human being needs around 1 gram of fat per 1 kilogram of body weight. This amount is smaller in children and elderly people and higher in people who have physically demanding jobs.

Experiment 2

Detecting fats in food.

You will need
  • tissue paper discs or paper tissues,

  • potato masher or hammer,

  • vegetable oil, sunflower seeds, nuts, flaxseed,

  • dropping pipette.

Instruction
  1. Check, whether the fat leaves an oily stain on the tissue paper (a stain that does not dry). Put a drop of oil onto the paper and observe the stain that is created.

  2. Between pieces of tissue paper put separately: sunflower seeds, flax seed, a piece of a nut without a shell. Crush them in a way that does not damage the tissue paper.

  3. After 2‑3 minutes throw away the crushed samples and observe how the tissue paper looks like. Compare this tissue paper with the one with oil on it.

Summary

If they leave fat stains, this means they included fats.

Comestible products (100 g)

Protein

Carbohydrates

Fats

Fibres

soy

35,9

15,8

18,3

15,7

Pasmesan cheese

35,6

25,8

Emmentaler cheese

28,7

0,2

29,7

4,6

lean pork

21,8

2,4

tuna

21,5

15,5

beef

20,6

8,1

turkey

20,6

6,9

trout

19,5

2,7

chicken

18

18,8

cream cheese

16,8

13

4,2

semi‑skimmed curd cheese

13,5

4

0,2

wholemeal pasta

12,6

59,9

3,6

3,5

buckwheat

12,6

70,1

3,1

5,9

chicken eggs

12,5

1

10,5

prunes

4,9

70,8

0,9

16

mushroom

2,7

0,7

0,3

1,9

spinach

2,5

0,6

0,3

1,8

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Exercise 1
Select the nutrients that can be found in seawater fish meat in high amounts. Możliwe odpowiedzi: 1. protein, 2. carbohydrates, 3. unsaturated fatty acids, 4. saturated fatty acids
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Exercise 2
Select the nutrients that provide most energy to the body. Możliwe odpowiedzi: 1. proteins, 2. carbohydrates, 3. fats, 4. fibre
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Exercise 3
Nutrients introduced into the body Możliwe odpowiedzi: 1. must be divided into smaller particles, 2. should connect with each other before digestion, 3. immediately reach the cells
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Exercise 4
Proteins Możliwe odpowiedzi: 1. are ingredients of muscle cells, 2. are digesting proteins (pepsin), 3. are composed of glycerol and fatty acids, 4. are a component of ATP
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Exercise 5
Backup sugar in our body is Możliwe odpowiedzi: 1. amino acid, 2. fatty acid, 3. glucose, 4. glycogen

Summary

  • Nutrients are organic compounds: proteins, sugars, fats, vitamins, and inorganic compounds – water and mineral salts.

  • Elements that provide energy are: sugars, fats and proteins (in extreme situations).

  • Building elements are: proteins, fats, mineral salts and water.

  • A healthy diet should be balanced and it should include, among other things, vegetable fats which are rich in unsaturated fatty acids.

  • Food that comes from animals provides us with exogenic amino acids.

Homework
Task 3.1

Present 3 arguments that confirm the thesis that every day diet should be rich in vegetables and fruit.

Keywords

proteins, carbohydrates, fats

Glossary

exogenous amino acids
exogenous amino acids
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Nagranie dźwiękowe słówka

aminokwasy egzogenne – aminokwasy, których organizm nie potrafi wytworzyć i musi je przyjmować z pokarmem w postaci gotowej do wykorzystania

endogenous amino acids
endogenous amino acids
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Nagranie dźwiękowe słówka

aminokwasy endogenne – aminokwasy, które organizm wytwarza z dostarczonych mu składników pokarmowych

fiber
fiber
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Nagranie dźwiękowe słówka

błonnik – zespół substancji pochodzenia roślinnego, zawierający m.in. celulozę, regulujący procesy trawienne

cholesterol
cholesterol
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Nagranie dźwiękowe słówka

cholesterol – składnik błon komórkowych, osłonek mielinowych nerwów; powstają z niego niektóre hormony; może odkładać się w naczyniach krwionośnych i wywoływać miażdżycę

glycerol
glycerol
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Nagranie dźwiękowe słówka

glicerol – związek organiczny, który może przyłączyć kwasy tłuszczowe i wraz z nimi utworzyć cząsteczkę tłuszczu; jego popularna nazwa to gliceryna

glycogen
glycogen
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Nagranie dźwiękowe słówka

glikogen – wielocukier zapasowy zwierząt; jest gromadzony w wątrobie i mięśniach

fatty acids
fatty acids
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Nagranie dźwiękowe słówka

kwasy tłuszczowe – związki organiczne, w skład których wchodzą m.in. łańcuchy węglowodorów; w reakcji z glicerolem tworzą tłuszcze; w komórkach pełnią funkcję energetyczną i zapasową

starch
starch
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Nagranie dźwiękowe słówka

skrobia – wielocukier zapasowy gromadzony przez rośliny

vitamins
vitamins
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Nagranie dźwiękowe słówka

witaminy – związki organiczne, które pełnią w organizmie funkcję regulacyjną